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Ԕ(ni)

|(zh)ٵø3(MMP3)ELISAzyԇ

cΔ(sh)285  l(f)rg2024/9/18

˾QϺhĽƼ޹˾ ̖48T/96T a(chn)ַЇ ѫ@c285 νB ţ|(zh)ٵø3(MMP3)ELISAzyԇa(chn)ƷHڿƌW(xu)о߹I(y)(yng)õȷt(y)ĿR\ίˎʳá
Ԕ(ni)
   ԇֻڿƌW(xu)оt(y)W(xu)\

 

ţ|(zh)ٵø3(MMP3)ELISAzyԇ

ʹf

zyԭ
ԇвpwһAķø“(lin)ԇ򞣨ELISAA(y)Ȱ|(zh)ٵø3(MMP3)wİ΢Уμ˱˜ƷHRPӛęzyw(jng)^صϴõTMB@ɫTMB^øĴ߻D(zhun){ɫD(zhun)ɽKSɫɫ\͘Ʒе|(zh)ٵø3(MMP3)P(gun)ø˃x450nm LœyȣOD ֵӋƷ




 

 

Ʒռ̎淽

1.  Ѫ壺ʹòԭ̓(ni)صԇ^бκμ̼ռѪҺ3000D(zhun)x10犌ѪͼtѸСĵطx

2.  Ѫ{EDTA}ؿ3000D(zhun)x30ȡ

3.  Һ3000D(zhun)x10ȥw;ۺ

4.  M{Mm}ˮv顣3000D(zhun)x10ȡ

5.  棺ӱռ󲻼rzyՈһb-20ⷴ(f)Ҝ½_Ʒسֽ
 

ԂƷ

ø˃x450nm

߾ȼӘ^0.5-10uL2-20uL20-200uL200-1000uL

37
 

ע

 ԇб2-8ʹҜƽ20ıȡĝsϴҺнY(ji)@F(xin)ˮԡӟʹY(ji)ȫܽʹ

 вõİl(yng)ŻԷܷ⣨͜ظ档

 Ȟ0S0̖˜ƷҕԌջ߿հfrӱѽ(jng)ϡ5KY(ji)5ǘӱH

 fИĕrgҺMМ

 ҺwMʹó֓u
 

ԇнM

Q

96

48

ע

΢ø˰

12×8l

12×4l

o

˜Ʒ

0.3mL*6

0.3mL*6

o

ӱϡҺ

6mL

3mL

o

zyw-HRP

10mL

5mL

o

20×ϴ쾏_Һ

25mL

15mL

fMϡ

A

6mL

3mL

o

B

6mL

3mL

o

KֹҺ

6mL

3mL

o

Ĥ

2

2

o

f

1

1

o

Է

1

1

o

ע˜ƷS0-S5Ξ飺02481632 nmol/L
 

ԇĜʂ

20×ϴ쾏_Һϡጣsˮ120ϡ1ݵ20×ϴ쾏_Һ19ݵsˮ
 

ϴ巽

 ֹϴ壺˦M׃(ni)Һwÿ׼ӝMϴҺo1min˦M׃(ni)Һwˮĸɣϴ5

 ԄϴCÿעϴҺ350μL1minϴ5
 

E

 Ҝƽ20minXȡlʣlԷܷŻ4档

 O(sh)ؘƷ׺͘ӱ˜Ʒ׸ӲͬȵĘ˜Ʒ50μL

 ӱȼӴyӱ10μLټӘӱϡҺ40μLհ׿ײ

 հ׿⣬˜Ʒ׺͘ӱÿ׼^øHRPӛęzyw100μL÷Ĥס(yng)37ˮԡ偻60min

 ȥҺwˮĸÿ׼ӝMϴҺo1min˦ȥϴҺˮĸɣ؏(f)ϴ5ΣҲϴCϴ壩

 ÿ׼AB50μL37ܹ15min

 ÿ׼KֹҺ50μL15min(ni)450nmL̎y׵ODֵ
 

Y(ji)Д

LƘ˜ExcelԘ˜ƷM(yng)ODֵvLƳ˜ƷԻؚwӋӱֵ

 

ԇ

ʴ_ԣ˜ƷԻؚwcA(y)ڝP(gun)ϵ(sh)Rֵڵ0.9900

`ȣ͙zyС0.1 nmol/L

خԣcԽY(ji)(gu)サ淴(yng)

؏(f)ԣ(ni)g׃ϵ(sh)С15%

Aأ2-8ܹ档

Чڣ6
 

؟(z)•

 ԇЃHоʹRw򞣬ta(chn)һкɌ߳Г(dn)˾Ųؓ؟(z)

 f`fɌ߳Г(dn)

 


 

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