Ԕ(ni)
ţ|(zh)ٵø3(MMP3)ELISAzyԇ
ʹf
zyԭ
ԇвpwһAķø(lin)ԇELISAA(y)Ȱ|(zh)ٵø3(MMP3)wİУμ˱˜ƷHRPӛęzyw(jng)^صϴõTMB@ɫTMB^øĴD(zhun){ɫD(zhun)ɽKSɫɫ\͘Ʒе|(zh)ٵø3(MMP3)P(gun)ø˃x450nm LyȣOD ֵӋƷ
Ʒռ̎淽
1. Ѫ壺ʹòԭ̓(ni)صԇ^бκμ̼ռѪҺ3000D(zhun)x10犌ѪͼtѸСĵطx
2. Ѫ{EDTA}ؿ3000D(zhun)x30ȡ
3. Һ3000D(zhun)x10ȥw;ۺ
4. M{Mm}ˮv顣3000D(zhun)x10ȡ
5. 棺ӱռrzyՈһb-20ⷴ(f)Ҝ½_Ʒسֽ
ԂƷ
ø˃x450nm
߾ȼӘ^0.5-10uL2-20uL20-200uL200-1000uL
37
ע
ԇб2-8ʹҜƽ20ıȡĝsϴҺнY(ji)@F(xin)ˮԡӟʹY(ji)ȫܽʹ
вõİl(yng)ŻԷܷ⣨͜ظ档
Ȟ0S0̖˜ƷҕԌջ߿հfrӱѽ(jng)ϡ5KY(ji)5ǘӱH
fИĕrgҺMМ
ҺwMʹó֓u
ԇнM
Q | 96 | 48 | ע |
ø˰ | 12×8l | 12×4l | o |
˜Ʒ | 0.3mL*6 | 0.3mL*6 | o |
ӱϡҺ | 6mL | 3mL | o |
zyw-HRP | 10mL | 5mL | o |
20×ϴ쾏_Һ | 25mL | 15mL | fMϡ |
A | 6mL | 3mL | o |
B | 6mL | 3mL | o |
KֹҺ | 6mL | 3mL | o |
Ĥ | 2 | 2 | o |
f | 1 | 1 | o |
Է | 1 | 1 | o |
ע˜ƷS0-S5Ξ飺02481632 nmol/L
ԇĜʂ
20×ϴ쾏_Һϡጣsˮ120ϡ1ݵ20×ϴ쾏_Һ19ݵsˮ
ϴ巽
ֹϴ壺˦M׃(ni)ҺwÿӝMϴҺo1min˦M׃(ni)Һwˮĸɣϴ5
ԄϴCÿעϴҺ350μL1minϴ5
E
Ҝƽ20minXȡlʣlԷܷŻ4档
O(sh)ؘƷ͘ӱ˜ƷӲͬȵĘ˜Ʒ50μL
ӱȼӴyӱ10μLټӘӱϡҺ40μLհײ
հ⣬˜Ʒ͘ӱÿ^øHRPӛęzyw100μL÷Ĥס(yng)37ˮԡ偻60min
ȥҺwˮĸÿӝMϴҺo1min˦ȥϴҺˮĸɣ؏(f)ϴ5ΣҲϴCϴ壩
ÿAB50μL37ܹ15min
ÿKֹҺ50μL15min(ni)450nmL̎yODֵ
Y(ji)Д
LƘ˜ExcelԘ˜ƷM(yng)ODֵvLƳ˜ƷԻؚwӋӱֵ
ԇ
ʴ_ԣ˜ƷԻؚwcA(y)ڝP(gun)ϵ(sh)Rֵڵ0.9900
`ȣ͙zyС0.1 nmol/L
خԣcԽY(ji)(gu)サ淴(yng)
؏(f)ԣ(ni)g׃ϵ(sh)С15%
Aأ2-8ܹ档
Чڣ6
؟(z)
ԇЃHоʹRwta(chn)һкɌ߳Г(dn)˾Ųؓ؟(z)
f`fɌ߳Г(dn)
ԭ(chung)ߣϺhĽƼ˾
Կ | |
dȤĮa(chn)Ʒ | * |
Ćλ | * |
* | |
(lin)ϵԒ | * |
Ԕַ | |
]䣺 | |
κҪҊh * |
|
Ca | * |
ՈݔҪP(gun)I~
ϺhĽƼ˾Һ,SùҺ,˰ELISAԇ,ELISAԇ,СELISAԇ,@ɫԇ,ţѪ,˜̥ţѪ,ţѪҺ,СѪ{,ut,|(zh)øҺ,B(yng),ȵøҺ,ùBҺ,LB_Һ,ABTSԇ,øƄ,EB_Һ,ɹȾɫԇ,ʴȾɫҺ,Դ|(zh),ىAҘ˜Ʒ,Ѽtؘ˜ƷPd-C-II˜Ʒ,֪ĸE˜Ʒ,B(yng),puc19|(zh),CPD,ɹ⿹wϡҺ
ַϺмζ^(q)ܰ·5588̖ Ԓ021-58999639 棺021-58999639 g(sh)֧֣xW(wng) L7290548